This enzyme will allow for removal of nucleic acids in a traditional protein buffer regime – ie the protein is well protected while the nucleic acids are fully removed. Digests DNA and RNA in approximately a 10:1 ratio. Applications in removal of contaminating nucleic acids from purified enzyme/protein preps as well as inline digestion of nucleic acids in protein purifications. The digestion can take place at high salt conditions.
Main advantages with Salt Active Nuclease
- Non specific endonuclease
- Optimum activity at high salt concentration (0.5 M NaCl)
- Active at low temperatures (20% at 6ºC)
- Broad pH range
- Temperature stable
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